The expression of cell cycle inhibitor p21 is altered by BMP4. (A) MCF-10A cells were treated with 100 ng/ml BMP4 (+) or vehicle (−) for 5 days and the cancer cell lines for 24 hours when grown as monolayers (2D). In Matrigel (3D), the cells were grown and treated for 4 (MDA-MB-361) or 7 days. The expression of p21 was analyzed by western blot. Tubulin was used as a loading control and relative expression levels were calculated with ImageJ. (B) BMP4 treatment leads to G1 arrest of MCF-10A cells. The cell cycle was determined by flow cytometry at day 5 after the beginning of the treatments. The fraction (mean + s.d.) of cells in phases G1, S and G2 are shown. **P < 0.01.
Ampuja et al. BMC Cancer 2013 13:429 doi:10.1186/1471-2407-13-429