BMP4 inhibits MCF-10A cell growth in 3D cell culture. Cells were grown in Matrigel (A-C) or in PEG gel (D-F) supplemented with 100 ng/ml BMP4 or vehicle. Images were captured with Olympus IX71 microscope and representative examples from day 14 (Matrigel, panel A) and day 11 (PEG gel, panel D) are shown. Scale bars 200 μm. (b, e) Cell proliferation was measured using the alamarBlue reagent at indicated time points and relative proliferation (mean + s.d.) compared to vehicle is presented. (C, F) The area covered by cell clusters was measured from images taken at indicated time points using ImageJ and the relative mean area and s.d. compared to vehicle is shown. *P < 0.05, **P < 0.01.
Ampuja et al. BMC Cancer 2013 13:429 doi:10.1186/1471-2407-13-429