Characterization of MCF-10A cells in 2D and 3D culture. (A) BMP4 treatment significantly reduces the proliferation of MFC-10A cells in 2D culture. Cells were grown in the presence of 100 ng/ml BMP4 or vehicle and proliferation was measured using the alamarBlue reagent and by counting the cells at indicated time points. Relative proliferation (mean + s.d.) compared to vehicle is shown. *P < 0.05, **P < 0.01. (B) MCF-10A cells form polarized structures in Matrigel but not in PEG gel. The cells were grown in Matrigel for 14 and in PEG gel for 11 days, fixed, and immunofluorescently labeled with polarization marker α6-integrin antibody (green). The nuclei were stained with DAPI (blue). Images were taken with Zeiss Axio Imager.M2 microscope. Scale bar 10 μm.
Ampuja et al. BMC Cancer 2013 13:429 doi:10.1186/1471-2407-13-429