Model of MM tumor induction and targeted chemotherapy in SCID mice. A schematic overview of the MM tumor induction protocol. Tumor formation was achieved by a single IP injection of MM (1 × 106 cells) followed by a 4 wk latency period to allow tumor growth. Mice were weighed every 3-4 days. The treatment groups included IP injections of saline (500 μL), APMS-MB (no DOX), DOX at a low dose (0.05 mg/kg), APMS-MB loaded with DOX at equivalent low dose (0.05 mg/kg), and DOX at a high dose (0.2 mg/kg) (all in saline at 500 μL, 3× per wk for 3 wks). Mice were euthanized after a total of 8 treatment injections due to the morbidity and weight loss in the DOX high group (N = 6 mice/group). Two mice in the saline group did not develop tumors, thus N = 4 in subsequent analysis of data.
Macura et al. BMC Cancer 2013 13:400 doi:10.1186/1471-2407-13-400