The effect of P276-00, doxorubicin and the combination of doxorubicin followed by P276-00 on cell proliferation, cell cycle and on recovery of cells after the treatment.(A) Cells were treated with 200 nM doxorubicin for 24 h or 800 nM of P276-00 for 72 h or combination of both. (B) Cells were treated with 100 nM doxorubicin for 24 h or 1200 nM P276-00 for 96 h or combination of both. (C) Percent of cell population in different phases of cell cycle. (D) Percent of cell population in sub-G1 phase after treatment with either drug alone or combination for 96 h and 120 h and (E) Recovery of cells after the drug treatment was studied at different timepoints i.e. 0 h, 6 h, 18 h, 24 h and 48 h. Cell cycle analysis using Flow cytometry shows the percentage apoptosis in drug alone and combination treatment in comparison to untreated control.
Rathos et al. BMC Cancer 2013 13:29 doi:10.1186/1471-2407-13-29