(A & B) The effect of P276-00 and doxorubicin treatment on growth of three NSCLC cell lines. Cells were seeded in 96-well plates and incubated overnight. P276-00 was added at the indicated concentrations and cells were further incubated for 48 h. Cell proliferation was determined using PI assay. (C &D) The 50% inhibitory concentrations of P276-00 and doxorubicin in three different NSCLC cell lines. (E) Effect of P276-00 and doxorubicin used singly or in combination on survival of H-460, H1299, H23 cell lines. The cells were treated as described under Materials and method section. There was significantly higher growth inhibition of the cells treated with doxorubicin (24 h) followed by P276-00 at IC50 concentration for 72 h compared to the cells treated with either agent alone.
Rathos et al. BMC Cancer 2013 13:29 doi:10.1186/1471-2407-13-29