Validation of DLL1 as a miR-34a target gene. (A) Computational algorithm showing the seed region of miR-34a at the 3’UTR of DLL1. (B) Western blotting analysis of the expressions of DLL1 and NOTCH1 upon miR-34a force-expression. (C) Functional luciferase assay. Significant differences was found between scramble and pre-miR-34a on wild-type 3’UTR construct but not with construct carrying a mutated seed region (n = 4). (D & E) Quantitative real-time PCR analysis showing the mRNA levels of DLL1, NOTCH1 (D) and Hes-1 (E) between pre-miR-34a and scramble precursor transfected cells (n = 4).*p < 0.05.
Pang et al. BMC Cancer 2013 13:25 doi:10.1186/1471-2407-13-25