Table 2

Murine anti-human PSA IgG2a titers in the serum of vaccinated human FcεRIα transgenic mice
Titers 14 days after initial immunization
Mouse # PSA + anti-PSA IgE PSA + anti-PSA IgG1 PSA Buffer
1 0 0 0 0
2 0 0 0 0
3 200 0 0 0
4 0 0 0 0
5 0 0 50 0
6 0 0 0 0
7 0 0 0 0
8 100 0 0 0
Median 0 0 0 0
Titers 13 days after booster (28 days after initial immunization)
Mouse # PSA + anti-PSA IgE PSA + anti-PSA IgG1 PSA Buffer
1 0 0 1,600 0
2 200 0 50 0
3 200 50 0 0
4 800 0 0 0
5 200 50 400 0
6 400 50 0 0
7 100 50 50 0
8 100 50 50 0
Median 200 50 50 0

Data shown are combined from two separate experiments (n = 4) with a total of 8 animals per group. Sera were serially diluted 2-fold starting at 1:50. Titer was determined as the highest dilution from which a positive signal was obtained.

Daniels-Wells et al.

Daniels-Wells et al. BMC Cancer 2013 13:195   doi:10.1186/1471-2407-13-195

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