Table 1

Murine anti-human PSA IgG1 titers in the serum of vaccinated human FcεRIα transgenic mice
Titers 14 days after initial immunization
Mouse # PSA + anti-PSA IgE PSA + anti-PSA IgG1 PSA Buffer
1 8,100 100 900 0
2 2,700 24,300 0 0
3 900 8,100 0 0
4 2,700 24,300 0 0
5 2,700 24,300 2,700 0
6 900 72,900 8,100 0
7 24,300 8,100 900 0
8 900 2,700 900 0
Median 2,700 16,200 900 0
Titers 13 days after booster (28 days after initial immunization)
Mouse # PSA + anti-PSA IgE PSA + anti-PSA IgG1 PSA Buffer
1 218,700 8,100 24,300 0
2 218,700 72,900 0 0
3 72,900 24,300 0 0
4 218,700 218,700 0 0
5 72,900 400,000 218,700 0
6 72,900 218,700 218,700 0
7 24,300 218,700 8,100 0
8 218,700 24,300 72,900 0
Median 145,800 145,800 16,200 0

Data shown are combined from two independent experiments (n = 4) with a total of 8 animals per group. Sera were serially diluted 3-fold starting at 1:100. Titer was determined as the highest dilution from which a positive signal was obtained.

Daniels-Wells et al.

Daniels-Wells et al. BMC Cancer 2013 13:195   doi:10.1186/1471-2407-13-195

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