Binding to antigen and FcεRIα. A) Binding of the anti-PSA IgE compared to a NS IgE to PSA was detected by ELISA. B) Binding to the known epitope recognized by AR47.47 was confirmed by ELISA using PSA peptides containing the antigen binding site. Binding was detected by an AP-conjugated anti-human κ secondary antibody followed by a phosphatase substrate. ELISA data are the mean of triplicate samples with standard deviations indicated and are representative of 3 independent experiments. C) Binding to the FcεRIα on the surface of CHO-3D10 cells was detected using flow cytometry and an anti-human κ FITC-conjugated antibody. An anti-FcεRI PE-conjugated antibody was used simultaneously to verify receptor expression. Data are representative of 3 independent experiments.
Daniels-Wells et al. BMC Cancer 2013 13:195 doi:10.1186/1471-2407-13-195