Cotreatment of Rsv and TMZ reduces clonogenic growth and induces senescence in glioma cells. U87 cells were treated with Rsv 30 μM, TMZ 100 μM or RT for 48 h, followed by two washes for drug removal. Then, (A) cells were plated for clonogenic assay; left – number of colonies after 14 days; right – representative images of colonies; scale bar: 100 μm; (B) cells were treated as above and plated for senescence measurement; left – percentage of SA-beta-gal-positive cells; right – representative images of treated cells; insert = SA-beta-gal-negative cells in TMZ-treated image. ** p<0.01 and *** p<0.001 in relation to control. (C) primary glioblastoma cells were treated as in above followed by growth for 7 days in DFM and analyzed by SA-beta-gal assay.
Filippi-Chiela et al. BMC Cancer 2013 13:147 doi:10.1186/1471-2407-13-147