Comparison of the effects of NVP-BEZ235 versus PP242 versus rapamycin on colon cancer cell growth, proliferation and apoptosis. LS174T, DLD-1 and SW480 colon cancer cells were treated with 10 nM of rapamycin, 100 nM of NVP-BEZ235 or 100 nM of PP242 for 48 hours. A: Cell growth was determined using a colorimetric MTS assay. Columns, mean cell growth relative to control of three independent experiments; bars, SD. B: Cell proliferation was assessed by BrDU incorporation. Results are quantified as percentage of positive cells for BrDU incorporation. Columns, mean percentage of three independent experiments; bars, SD C: Cell apoptosis was evaluated using a cell death detection ELISA. Cells were harvested and apoptosis was measured by quantifying DNA fragmentation. Columns, mean enrichment factor at 405 nm of three independent experiments; bars, SD. *, P < 0.05, compared to control or otherwise as specified by brackets.
Blaser et al. BMC Cancer 2012 12:86 doi:10.1186/1471-2407-12-86