Effects of rapamycin, NVP-BEZ235 and PP242 on mTORC1 and mTORC2 activity in colon cancer cells. LS174T, DLD-1 and SW480 colon cancer cells were treated with increasing concentrations of rapamycin, NVP-BEZ235 or PP242 for six hours. Cells were lysed and analyzed by Western blot for phospho-S6 ribosomal protein and phospho-Akt as a read-out for mTORC1 and mTORC2 activity respectively. Total S6 ribosomal protein and total Akt were used as loading controls. The illustrated blots are representative of three independent experiments.
Blaser et al. BMC Cancer 2012 12:86 doi:10.1186/1471-2407-12-86