Additional file 1.
Figure S1. RNAi transfection decreases PLK1 mRNA and protein levels in medulloblastoma cell lines. Quantifying bar graphs depicting significant decreases in PLK1 mRNA levels in Daoy medulloblastoma cell line transfected: (A) with either a non-targeting shRNA (shNTC) or two different shRNAs targeting PLK1 (shPLK1 1073 and shPLK1 1325), (B) with either a scrambled siRNA (siRNA N.C.) or three different siRNAs targeting PLK1 (siPLK1-A, siPLK1-B, and siPLK1-C). Error bars represent SEM. (C) ONS-76 cells show a significant decrease in PLK1 mRNA expression following transfection with siPLK1-A. Error bars represent SEM. (B) Western blot analysis confirms that RNAi-mediated knockdown of PLK1 decreases the level of PLK1 protein in both Daoy and ONS-76 cells.
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Harris et al. BMC Cancer 2012 12:80 doi:10.1186/1471-2407-12-80