Figure 3.

Increased apoptosis following PLK1 mRNA inhibition by RNAi. (A) Medulloblastoma cell lines Daoy and ONS-76 were transfected with either a non-silencing siRNA (siRNA N.C.) or a siRNA targeting PLK1 (siPLK1-A) for 72 hours and stained with Guava Nexin reagent. Representative flow cytometry plots for the two cell lines are shown. Percentages of cells in the bottom left quadrant are live while cells to the right of the vertical line are apoptotic or dead. (B) Quantifying bar graphs showing a significant increase in the percentage of apoptotic/dead cells following PLK1 mRNA knockdown with siRNA in both Daoy and ONS-76 cell lines. Error bars represent SEM

Harris et al. BMC Cancer 2012 12:80   doi:10.1186/1471-2407-12-80
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