Open Access Research article

Opposing function of MYBBP1A in proliferation and migration of head and neck squamous cell carcinoma cells

Gustavo A Acuña Sanhueza123, Leonie Faller1, Babitha George1, Jennifer Koffler1, Vinko Misetic1, Christa Flechtenmacher4, Gerhard Dyckhoff1, Peter P Plinkert1, Peter Angel3, Christian Simon1 and Jochen Hess12*

  • * Corresponding author: Jochen Hess

  • † Equal contributors

Author Affiliations

1 Department of Otolaryngology, Head and Neck Surgery, University Hospital Heidelberg, 69120 Heidelberg, Germany

2 Junior Research Group Molecular Mechanisms of Head and Neck Tumors, German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, 69120 Heidelberg, Germany

3 Division of Signal Transduction and Growth Control, German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, 69120 Heidelberg, Germany

4 Institute of Pathology, University Hospital Heidelberg, 69120 Heidelberg, Germany

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BMC Cancer 2012, 12:72  doi:10.1186/1471-2407-12-72

Published: 17 February 2012

Additional files

Additional file 1:

Table S1. Primers used for RQ-PCR.

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Additional file 2:

Figure S1. Proliferation characteristics of primary and recurrent mouse tumors. (A) Ki67 protein expression was analyzed by immunohistochemistry on tumor sections derived from the surgical mouse model used in this study. A prominent nuclear staining was observed both in primary tumors and respective recurrence. (B) Quantification of positive nuclei revealed a significant decrease in the amount of Ki67-positive cells in recurrent compared to primary tumors (p-value: 0.0004). Scale bars, 50 μm.

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Additional file 3:

Figure S2. Invasive capacity comparison of SCC-7, Cal-27 and SCC-25. The invasiveness of the cell lines was assessed in Boyden chambers. SCC-25 shows a higher invasive capacity than the high MYBBP1A expressing cell lines Cal-27 and SCC-7 (p-value: 0.014) (A).

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