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Open Access Research article

PINCH is an independent prognostic factor in rectal cancer patients without preoperative radiotherapy - a study in a Swedish rectal cancer trial of preoperative radiotherapy

Annica Holmqvist1*, Jingfang Gao1, Birgitta Holmlund1, Gunnar Adell1, John Carstensen2, Dianne Langford3 and Xiao-Feng Sun1

Author Affiliations

1 Department of Medical Oncology, Institute of Clinical and Experimental Medicine, Linköping University, S-58185 Linköping, Sweden

2 Institute of Health and Society, Faculty of Health Sciences, Linköping University, Linköping, Sweden

3 Department of Neuroscience, Temple University School of Medicine, Philadelphia, PA, USA

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BMC Cancer 2012, 12:65  doi:10.1186/1471-2407-12-65

Published: 10 February 2012

Abstract

Background

The clinical significance between particularly interesting new cysteine-histidine rich protein (PINCH) expression and radiotherapy (RT) in tumours is not known. In this study, the expression of PINCH and its relationship to RT, clinical, pathological and biological factors were studied in rectal cancer patients.

Methods

PINCH expression determined by immunohistochemistry was analysed at the invasive margin and inner tumour area in 137 primary rectal adenocarcinomas (72 cases without RT and 65 cases with RT). PINCH expression in colon fibroblast cell line (CCD-18 Co) was determined by western blot.

Results

In patients without RT, strong PINCH expression at the invasive margin of primary tumours was related to worse survival, compared to patients with weak expression, independent of TNM stage and differentiation (P = 0.03). No survival relationship in patients with RT was observed (P = 0.64). Comparing the non-RT with RT subgroup, there was no difference in PINCH expression in primary tumours (invasive margin (P = 0.68)/inner tumour area (P = 0.49). In patients with RT, strong PINCH expression was related to a higher grade of LVD (lymphatic vessel density) (P = 0.01)

Conclusions

PINCH expression at the invasive margin was an independent prognostic factor in patients without RT. RT does not seem to directly affect the PINCH expression.

Keywords:
PINCH; Radiotherapy; Prognosis; Rectal cancer; Immunohistochemistry