Additional file 5: Figure S5..
Representative flow cytometry dot plot for defining viable CECs and apoptotic CECs, (A) Exclude debris and red blood cells. (B) CEC and CEPs are within CD45dim population (P2). (C)CD146 was CEC maker and 7AAD staining was used to identify the cell viability. Cells in Q2 are apoptotic CECs and in Q4 are viable CECsRepresentative data of dynamic change of CEC, CEP (S-Figure 5a, 5c, 5e and 5 g) and viable-CEC (S-Figure 5b, 5d, 5f and 5 h) levels during second cycle of chemotherapy from one patient. CEC, CEP (S-Figure 5a) and viable-CEC (S-Figure 5b) at the day before chemotherapy (for this patient, taxotere /epirubicin /cyclophosphamide) were shown in S-Figure 5a and 5b. Patient’s CEC, CEP and viable-CEC levels were dropping at day 4 and day 7 (S-Figure 5c, 5d, 5e and 5f) after chemotherapy. Three weeks after chemotherapy, CEC and CEP levels were increased again(S-Figure 5e) and most of the CECs were viable (S-Figure 5f).
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Kuo et al. BMC Cancer 2012 12:620 doi:10.1186/1471-2407-12-620