Neural protein gamma-synuclein interacting with androgen receptor promotes human prostate cancer progression
- Equal contributors
1 Department of Urology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, China
2 Department of Urology, the Second Affiliated Hospital of Fujian Medical University, Quanzhou, China
3 Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai, China
BMC Cancer 2012, 12:593 doi:10.1186/1471-2407-12-593Published: 11 December 2012
Additional file 1: Figure S1:
Western blot analysis was used to evaluate SNCG protein silence efficiency in CWR22 cells with different siSNCG-166 concentration.
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Additional file 2: Figure S2:
Western blot analysis were used to detected the MMP members expression level with different SNCG expression state.
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Additional file 3: Figure S3:
Cell cycles analyzed by flow cytometry. Up-regulation of SNCG expression in LNCaP promoted cells into S phase compared to REP-NC.
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Additional file 4: Figure S4:
RT-PCR (A) and western blot (B) showed that the AR expression was higher in LNCaP-AI cells than in LNCaP cells **P<0.01.
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Additional file 5: Figure S5:
Extracted from “Xu B, Sun Y, Tang G, Xu C, Wang L, Zhang Y, Ji J: Id-l expression in androgen-dependent prostate cancer is negatively regulated by androgen through androgen receptor. Cancer letters 2009, 278: 220-9”.
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Additional file 6: Figure S6:
The stably transfected cell lines were constructed. The fluorescence image (up) and the protein level (down) were presented.
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Additional file 7: Figure S7:
SNCG protein expression at indicated time points in LNCaP cells that were transfected with SNCG plasmid (A) or siRNA (B).
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