Pattern and intensity of staining for potential cancer stem cell markers in hepatocellular carcinoma specimens. Formalin-fixed paraffin-embedded human HCC samples were immunostained for CD90, CD44, CD133, and EpCAM and intensity of staining was assessed as described in methods. For each marker, example images are shown demonstrating the staining pattern for each of the intensity grades.
Zeng et al. BMC Cancer 2012 12:584 doi:10.1186/1471-2407-12-584