Methylation of the PLA2R1 gene in bone marrow aspirates of MDS patients in dependence on IPSS classification. After isolation and subsequent bisulfite modification of genomic DNA from bone marrow aspirates of MDS patients (N = 38) methylations of amplified PLA2R1 sequences covering 5`-CpG sites 6–14 (−437 bp to −270 bp from exon 1) were analyzed using MS-HRM (HRM,%). Low, low risk; int-1, intermediate-1-risk; int-2, intermediate-2-risk; and high, high-risk classifications.
Menschikowski et al. BMC Cancer 2012 12:576 doi:10.1186/1471-2407-12-576