Methylation of the PLA2R1 gene in DNA samples from blood of patients with leukemia in comparison to normal individuals. MS-HRM analyses using RotorGene Q of amplified PLA2R1 sequences covering 5`-CpG sites 6–14 (−437 bp to −270 bp from exon 1) after isolation and subsequent bisulfite modification of genomic DNA from normal subjects (N1-N10, black), and patients with leukemia (P1 to P6, P9, and P10; in colors, individual data are shown in Additional file 1: Table S1). Difference plots normalized to the 0%-methylated standard DNA sample and a standard curve with 0%, 20%, 30%, 40%, 60%, and 100% methylation ratios in black dotted lines are shown.
Menschikowski et al. BMC Cancer 2012 12:576 doi:10.1186/1471-2407-12-576