Additional file 1.

Effect of GNF-2 and Abl kinase Inhibitors (AKIs) on the proliferation and clonigenicity of Ba/F3 cells.Figure S1: Effect of Imatinib and Dasatinib on the proliferation of Ba/F3 (Blue) and Ba/F3 p185 Bcr-Abl (Red) cells. The cells supplemented with 10 ng/ml of IL-3, were grown for 72 h in the presence of different Imatinib and Dasatinib concentrations. Cells were counted and percent of inhibition was calculated in relation to the solvent (0.5% DMSO) treated samples. Experiments were carried out in duplicates and repeated twice with comparable outcome. Figure S2: Cooperation between GNF-2 with Imatinib and Dasatinib in regulating proliferation of Ba/F3 cells. Ba/F3 supplemented with 10 ng/ml of IL-3 were grown for 72 h in the presence of various GNF-2 concentration (0.1, 0.5, 2.5, 5, 25 and 125 μM) alone or in combination with Imatinib (1 μM) and Dasatinib (50 nM). After 72 h incubation, cells were counted and percent of inhibition was calculated in relation to the solvent (0.5% DMSO) treated samples. Experiments were carried out in duplicates and repeated twice with comparable outcome. Figure S3: Effect of GNF-2 and AKIs on the clonigenicity of Ba/F3 cells. Ba/F3 cells grown on soft agar were treated with solvent (DMSO 0.5%; Un), Imatinib (1 μM), Dasatinib (1 μM), and GNF-2 (5 μM-100 μM) alone or in the presence of either 1 μM Imatinib or 1 μM Dasatinib. Experiments were carried out in duplicates and repeated twice with comparable outcome.

Format: DOCX Size: 1.6MB Download file

Khateb et al. BMC Cancer 2012 12:563   doi:10.1186/1471-2407-12-563