Effect of GNF-2 and AKIs on pBcr-Abl, pSTAT5α and pJAK2 in Ba/F3p185 Bcr-Abl T315I exposed to IL-3=(10 ng/ml). Ba/F3 cells carrying p185 Bcr-Abl T315I were exposed to IL-3 (10 ng/ml) and GNF2 (10 μM and 50 μM) alone or in combination with Imatinib (1 μM), Nilotinib (0.1 μM) and Dasatinib (0.1 μM) and levels of Bcr-Abl, pBcr-Abl, pJAK2, pSTAT5α and α-tubulin were determined by immune-blotting as previously described. Relative values of pBcr-Abl, pJAK2 and pSTAT5α relative to α-tubulin are shown.
Khateb et al. BMC Cancer 2012 12:563 doi:10.1186/1471-2407-12-563