Memory T cell responses against GBM peptide antigens detected by ELISPOT. The MAGE-A3 peptides (MAGE-A3112-127, MAGE-A3121-136, and MAGE-A3143-160 were dissolved in DMSO in equimolar amounts (peptide pool I) while the IL13Rα2 peptides IL13Rα2341-355 and IL-13Rα2351-365 were similarly dissolved together (peptide pool II) and used to stimulate freshly isolated PBMCs from 3 patients with primary GBMs. Significant (p < 0.05) responses to both peptide pools were detected in all patients. Mean + SD are presented.
Shimato et al. BMC Cancer 2012 12:561 doi:10.1186/1471-2407-12-561