(A) Apoptosis assay at 72 h after FGF19 recombinant protein treatment. (left) 5-FU treatment; (right) 5-FU and recombinant protein treatment. The apoptosis index (AI) was defined as the OD values of cells treated with these agents divided by those of untreated cells. (B) (upper) Invasion assay after FGF19 recombinant protein treatment (100× magnification). (lower) Invasion assay 48 h after FGF19 recombinant protein treatment after FGF19 recombinant protein treatment. (C) FGF19 enhanced cell migration ability in JHH7 cell line. The transwell system was used to evaluate migratory ability. Migrated cells were counted and the quantitative results are shown (n = 6). The data presented are from a representative experiment, being quantitatively similar in the replicate experiments.
Miura et al. BMC Cancer 2012 12:56 doi:10.1186/1471-2407-12-56