Additional file 1.

Reproducibility of multiplex qMSP. Serial dilutions of methylated DNA (SiHa) spiked with unmethylated DNA (EK) in order to obtain a serial dilution of 50 ng to 0.25 ng methylated DNA in a total of 50 ng of DNA, showed high reproducibility when testing the multiplex qMSP in 10-fold.

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Snellenberg et al. BMC Cancer 2012 12:551   doi:10.1186/1471-2407-12-551