Correlation between the ΔCq values obtained with singleplex and multiplex qMSP. The R2 values for the three markers were 0.985, 0.986 and 0.944 for CADM1 (A), MAL (B) and hsa-miR-124-2 (C), respectively. The ΔCq values between singleplex and multiplex qMSP differ, due to differences in set thresholds.
Snellenberg et al. BMC Cancer 2012 12:551 doi:10.1186/1471-2407-12-551