High linearity between singleplex and multiplex analysis on serial dilutions of methylated DNA (SiHa) in unmethylated DNA (EK). The multiplex reactions showed comparable efficiencies as the singleplex reactions for CADM1 (A), MAL (B) and hsa-miR-124-2 (C). Same thresholds were used in singleplex and multiplex qMSP, resulting in different Cq values for all targets. When adjusting the thresholds, the same Cq values can be obtained, resulting in overlap of the standard curves.
Snellenberg et al. BMC Cancer 2012 12:551 doi:10.1186/1471-2407-12-551