Effects of siRNA targeting PLK1 on growth, apoptosis and cell cycle of NSCLC cells. (A) RT-PCR and Western Blot analysis of PLK1 mRNA and protein expression in A549-siRNA/PLK1 or A549-siRNA/control cells. (B) MTT analysis of growth in A549 cells at different time points (0, 24, 48, 72 or 96h) after transfection with siRNA/PLK1 or siRNA/control. (C) Hoechst staining analysis of apoptosis in A549/miR-100 or A549/miR-NC cells. The percentage of Hoechst-positive nuclei per optical field (at least 50 fields) was counted. (D) Flow cytometric analysis of cell cycle distribution in A549-siRNA/PLK1 or A549-siRNA/control cells. * and ** indicate P < 0.05 and P < 0.01, respectively. Each experiment was performed at least in triplicate. Corresponding P values analyzed by t-tests are indicated.
Liu et al. BMC Cancer 2012 12:519 doi:10.1186/1471-2407-12-519