PLK1 is a direct target of miR-100. (A) Alignment between the predicted miR-100 target sites and miR-100 is shown. (B) RT-PCR and Western Blot assays were performed to detect the expression of PLK1 mRNA and protein expression in A549 cells transfected with miR-100 mimics (miR-NC mimics) or anti-miR-100 (anti-miR-NC). (C) A549 cells were co-transfected with miR-100 mimics or anti-miR-100 and pLUC vector with PLK1 3’-UTR-wild or mut. After 24 hours, the luciferase activity was measured. Values are presented as relative luciferase activity after normalization to Renilla luciferase activity. * and ** indicate P < 0.05 and P < 0.01, respectively. The data are expressed as the mean value ± SEM of the results obtained from three independent experiments. Corresponding P values analyzed by t-tests are indicated.
Liu et al. BMC Cancer 2012 12:519 doi:10.1186/1471-2407-12-519