Figure 2.

Metformin blocks cell cycle progression at the G0/G1 phase. Human OSCC cells (CAL27, WSU-HN6, and SCC25) were grown in 6-well plates (2 × 105 cells/well). After 24 h, the culture medium was removed and replaced with fresh culture medium containing 0 mM or 20 mM metformin for an additional 24 h. (A) Cell cycle progression in OSCC cells was assessed by flow cytometry. (B) The expression of related cell-cycle regulatory proteins in arrested and proliferating OSCC cells treated with or without metformin was assessed by immunoblotting. One representative experiment out of three is shown.

Luo et al. BMC Cancer 2012 12:517   doi:10.1186/1471-2407-12-517
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