Effect of sampling method on the expression of inflammatory and hypoxic stress responsive genes in healthy colon tissue samples. Relative mRNA expression levels of the inflammatory eicosanoid enzymes COX2 (A) and 5-LOX (B) and of the hypoxia markers GLUT-1 (C) and CAIX (D) are shown for healthy colon biopsies and healthy colon resection samples. Expression levels were normalized against reference genes TBP and SDHA and were scaled against the median of the biopsy samples (median set to 1). Expression data are depicted as scatter plots of the values obtained for each individual sample. The horizontal line represents the median; ns: not significant; *: p < 0.05; **: p < 0.01; ***: p < 0.001 with Mann–Whitney U Test.
Pringels et al. BMC Cancer 2012 12:515 doi:10.1186/1471-2407-12-515