The primers of MS-HRM and MSP assays for CST6 promoter methylation. The MS-HRM primers are shown in red. The outer MSP primers are framed, while the inner ones are shown in purple. The region from −162 up to +188 is depicted (+1 is the Transcriptional start site). Note that this sequence is produced after bisulfite conversion of genomic DNA. All CpGs are considered to be methylated, and therefore are unaffected during the conversion process.
Dimitrakopoulos et al. BMC Cancer 2012 12:486 doi:10.1186/1471-2407-12-486