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Phospho-specific flow cytometry identifies aberrant signaling in indolent B-cell lymphoma

Egil S Blix12*, Jonathan M Irish34, Anne Husebekk5, Jan Delabie6, Lise Forfang7, Anne M Tierens6, June H Myklebust7 and Arne Kolstad8

Author Affiliations

1 Department of Oncology, University Hospital of North Norway, Tromsø, Norway

2 Immunology Research group, Institute of Medical Biology, University of Tromsø, Tromsø, Norway

3 Department of Medicine, Oncology Division, Stanford University, Stanford, CA 94305, USA

4 Department of Microbiology and Immunology, Baxter Laboratory of Genetic Pharmacology, Stanford University, Stanford, CA 94305, USA

5 Department of Laboratory Medicine, University Hospital North Norway, Tromsø, Norway

6 Department of Pathology, Division of Cancer Medicine and Surgery, Oslo University Hospital, Oslo, Norway

7 Institute for Cancer Research, Oslo University Hospital and Centre for Cancer Biomedicine, Oslo, Norway

8 Department of Oncology, Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway

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BMC Cancer 2012, 12:478  doi:10.1186/1471-2407-12-478

Published: 16 October 2012

Additional files

Additional file 1:

Figure S1. No difference in anti-BCR induced signaling between CD20+CD5-and CD20+CD5+B cells. PBMCs (n=4) from healthy donors were stimulated with aBCR for 4, 15 or 45 minutes. Flow cytometry analysis of p-SFKs, p-SYK, p- PLCγ and p-S6 after gating on CD20+CD5+ or CD20+CD5- cells. (A) MFI in aBCR stimulated cells relative to unstimulated cells from the same subset is illustrated as heatmap. (B) Bar charts with median relative MFI ± SEM, n=4 healthy donors.

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