Figure 1.

Invasive ability enhanced by vincristine.A. The number of invading cells measured by the invasion assay. Cells (1 × 10⁶) were seeded into the upper chamber in the presence or absence of anti-cancer drugs. After 24 h incubation, the invading cells were fixed, and stained by toluidine blue. Total numbers of the stained cells were counted using a microscope. Con, non-treated control; Vin, vincristine; Pac, paclitaxel; Cis, cisplatin (15 μM); Eto, etoposide (20 μM). The graph shows mean ± S.E. of three independent experiments. *, P < 0.05; **, P < 0.01 versus Con. B. Cells (1 × 10⁴) were seeded into wells of a 96-well plate in the presence or absence of anti-cancer drugs. After 24 h incubation and the following 1 h incubation with WST-1 solution, the absorbance at 450 nm was recorded using a microplate reader. Cell viability is expressed as percentages relative to the viability obtained for non-treated control. Con, non-treated control; Vin, vincristine; Pac, paclitaxel; Cis, cisplatin (15 μM); Eto, etoposide (20 μM). The graph shows mean ± S.E. of three independent experiments. N.S., not-significant.