Morphological changes after treatment with Salinomycin. 3 x 104 Mz-ChA-1 and EGI-1 cells were plated in chamber slides and further cultured until confluence. Cells were subsequently incubated in the absence or presence of 1 mM Gemcitabine, 1 μM, 2 μM, 5 μM or 10 μM Salinomycin for 24 hours. (A): Effects of Gemcitabine and Salinomycin treatment on Mz-ChA-1 cells. Incubation of cells with 1 mM Gemcitabine resulted in swollen and cobblestone-like appearance. In contrast the morphology of Mz-ChA-1 cells after treatment with low concentration of Salinomycin is altered to a haggard image and less confluent cell growth dependent on the amount of added agent. This appearance was further enhanced by higher concentration of 5 μM and 10 μM Salinomycin. (B): Treatment of EGI-1 cells with Salinomycin even with 5 μM and 10 μM revealed increased tolerance of agent since indication of morphological damages were less distinct. 1 mM Gemcitabine however caused comparable outcome on EGI-1 cells compared to Mz-ChA-1 and TFK-1 cells. Results are shown as representative pictures of one out of 3 independent experiments.
Lieke et al. BMC Cancer 2012 12:466 doi:10.1186/1471-2407-12-466