Establishment of high invasive subclones and low invasive subclones. (A) The proliferations of high invasive subclones HeLa-1 and SiHa-1 and low invasive subclones HeLa-25 and SiHa-23 as examined by MTT assay. (B) The images of cells crossing PVPF filters as examined by cell migration assay using Boyden chambers. (C) The images of cells invading Matrigel-coated membranes as examined by cell invasion assay using Boyden chambers. (Magnification ×200). *P<0.05 versus control.
Chen et al. BMC Cancer 2012 12:464 doi:10.1186/1471-2407-12-464