RNA input range and reproducibility of EndoPredict. (A) EP scores depending on amount of input RNA. RNAs from 6 different FFPE samples were diluted and EP scores were assessed dependent on RNA input (Cq-ARG as surrogate marker). 95% confidence intervals (CI) of EP scores calculated from the noise model are indicated. (B) Correlation between Cq-ARG and total RNA concentration assessed by RIBOGREEN assay. Lower RNA input limit is indicated by dotted lines. (C) Reproducibility of 160 EP scores assessed in three different RNA samples (low risk, close to the decision point, high risk) over time (11 different working days distributed over 21 calendar days). Individual EP measurement results are indicated by dots.
Kronenwett et al. BMC Cancer 2012 12:456 doi:10.1186/1471-2407-12-456