Conditioned mediums from melanoma cell lines enhance transcript expression of ICAM-1 in HUVEC cells. Semi-quantitative PCRs were performed to detect expression of ICAM-1 transcripts. A HUVEC cells were treated either with TNF-α and IFN-γ at 100ng/ml or B with conditioned medium from A375 (H+A375), SLM8 (H+SLM8) and 1205LU (H+1205LU) after 48hrs of cell culture. GAPDH is used as a DNA amount control. Data were obtained from 3 independent experiments.
Ghislin et al. BMC Cancer 2012 12:455 doi:10.1186/1471-2407-12-455