Cytokine expression in co-cultures of NK cells and monocytes in the presence or absence of PstS-1. Co-cultures of NK cells and monocytes were stimulated with or without PstS-1 for one and three days. The expression of IL-12, IL-15, IL-18 and IFN-γ was analysed by quantitative RT-PCR. Samples from three independent experiments and donors were pooled. The cytokine expression of the stimulated co-culture (NK + Mo + PstS-1) is shown in relation to the unstimulated (NK + Mo) on day three (a). Corresponding to mRNA-expression cytokine protein levels of the samples were determined by BioPlex assay. Cytokine concentrations of the three independent experiments on day three are shown as means + SE in (b).
Gottschalk et al. BMC Cancer 2012 12:451 doi:10.1186/1471-2407-12-451