Curcumin-induced effects on APC/C and other cell cycle related proteins. A, Expression of the APC/C subunit APC2, the APC/C co-activator p55Cdc20 and cyclins A and E in control and curcumin-treated DAOY cells as determined by immunoblotting. GAPDH levels are included to ensure equal loading. B, Immunoblot of the APC/C subunit Cdc27 showing a curcumin-induced shift in MW (arrows) in unsynchronized DAOY cells. Because of differences in band intensity the same immunoblot is shown with two different exposures. Arrowhead and asterisk indicate the non-phosphorylated and phosphorylated bands of Cdc27, respectively C, Comparison of MW shift by curcumin between Cdc27 and other APC components. Tubulin was used for equal loading control.
Lee and Langhans BMC Cancer 2012 12:44 doi:10.1186/1471-2407-12-44