Open Access Research article

Evidence for different mechanisms of ‘unhooking’ for melphalan and cisplatin-induced DNA interstrand cross-links in vitro and in clinical acquired resistant tumour samples

Victoria J Spanswick1, Helen L Lowe1, Claire Newton1, John P Bingham1, Alessia Bagnobianchi1, Konstantinos Kiakos1, Charles Craddock2, Jonathan A Ledermann3, Daniel Hochhauser1 and John A Hartley1*

Author Affiliations

1 CR-UK Drug-DNA Interactions Research Group, UCL Cancer Institute, Paul O’Gorman Building, 72 Huntley Street, London, WC1E 6BT, UK

2 Centre for Clinical Haematology, Queen Elizabeth Hospital, Birmingham B15 2TH, UK

3 CR-UK/UCL Cancer Clinical Trials Centre, London, UK

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BMC Cancer 2012, 12:436  doi:10.1186/1471-2407-12-436

Published: 28 September 2012

Additional files

Additional file 1:

Table S1. GI50 values (dose of drug that inhibits growth by 50%) for melphalan and cisplatin in the human A548, RPMI8226 cell lines. Drug exposure was for 1 hour at 37°C and cells were incubated in drug-free medium for a further 4 days prior to analysis using the sulforhodamine B assay. Values are mean±s.d. from at least three independent experiments.

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