Effects of GNF-2 and Dasatinib on Rat-1 cells expressing BCR/ABL-T315I. (A) Transformation assays measuring colony formation in soft agar. Rat-1 cells were retrovirally transduced with the BCR/ABL-T315I construct and seeded at 104 cells/well on soft agar in six-well plates in the presence of 2.5 or 5 μM GNF-2 and 300 nM Dasatinib. After 15 days, the colonies were counted. The means +/- SD of triplicates of two representative experiments are presented. (B) Focus formation assays. A total of 4x104 infected Rat-1 cells were plated per well in 24-well plates, grown for 72 h to confluence and incubated for 12 additional days in the presence of 2.5 or 5 μM GNF-2 and 300 nM Dasatinib. The plates were then washed, dried and stained with crystal violet. One representative of each of the two experiments performed in triplicate is given (34X magnification).
Mian et al. BMC Cancer 2012 12:411 doi:10.1186/1471-2407-12-411