Figure 4.

Flow cytometric determination of DNA content in shCDCA3- and mock-transfected cells. A) Representative FACS analysis shows that the number of cells in the G1 phase is significantly (*p < 0.05, Mann-Whitney’s U test) increased in shCDCA3-transfected H1 and Sa3 cells. B) Western blot analysis of CDKIs (p21Cip1 p27Kip1, p15INK4B, and p16INK4A), CDK4, CDK6, Cyclin D1, and Cyclin E shows up-regulation of p21Cip1, p27Kip1, p15INK4B, p16INK4A, CDK4, and Cyclin D1, and down-regulation of CDK6 and Cyclin E in the CDCA3 knockdown cells

Uchida et al. BMC Cancer 2012 12:321   doi:10.1186/1471-2407-12-321
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