Additional file 2.

Figure S1. (A) Cell morphology (shape) of fascin-overexpressed and vector control clone analyzed by phase contrast microscopy. Scale bar: 100 μm. (B) Western blot analysis of fascin-overexpressed (AW-Fascin-1 and AW-Fascin-2) and vector control clones (AW-GFP-Cont) with antibody to vimentin. β-actin was used as a loading control. (C) Representative confocal images of b-catenin and E-cadherin staining in stable AW-Fascin-1, AW-Fascin-2 and AW-GFP-Cont clones. Scale bars: 10 μm. (D) Representative image of the size and number of colonies formed in soft agar of the indicated clones. Scale bar: 100 μm.

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Alam et al. BMC Cancer 2012 12:32   doi:10.1186/1471-2407-12-32