Additional file 3 Figure S3.

Serum-deprived MCF-7 cells do not secrete autocrine factors. The cells were made quiescent in medium with ICI 182780 during 48 h. They were then placed for 6 h in fresh medium (serum- and phenol red-free) with ICI 182780; this was used as conditioned medium (CM). Another series of dishes were stimulated for 1 h or 3 h with CM or with insulin as a positive control, lysed and analyzed for phospho-Ser473 Akt. (PPT 100 kb)

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Gaben et al. BMC Cancer 2012 12:291   doi:10.1186/1471-2407-12-291