Additional file 1 Figure S1.
Knock-down of the Akt signal by shAkt. Transfections were carried out by the Icafectin method (Eurogentec) according to the manufacturer’ protocol with the shRNA as indicated. The cells were serum-starved during 48h and then harvested and analyzed by Western blotting with the Akt antibody. Actin was used as control. (PPT 114 kb)
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Gaben et al. BMC Cancer 2012 12:291 doi:10.1186/1471-2407-12-291