ZIC1 inhibits the expression of Sonic hedgehog (Shh). (A) The expression of Shh protein was analyzed by Western blot in BGC823 and SGC7901 cells stably transfected with pCDNA3.1-ZIC1 or pCDNA3.1 empty vector. (B) The expression level of Shh mRNA was determined by real-time quantitative RT-PCR analysis. The relative expression level was expressed as fold change (mean ± SEM) compared with pCDNA3.1 empty vector normalized to 1.
Zhong et al. BMC Cancer 2012 12:290 doi:10.1186/1471-2407-12-290