ZIC1 modulates cell-cycle distributions and cell migration through regulation of sonic hedgehog, PI3K and MAPK signaling pathways in gastric cancer
- Equal contributors
1 Department of Gastroenterology, Second Affiliated Hospital, School of Medicine Zhejiang University, 88 Jiefang Road, Hangzhou, 310009, China
2 Laboratory of Digestive Disease, Sir Run run Shaw Clinical Medicine Institution of Zhejiang University, 3 Qingchun Road, Hangzhou, 310053, China
3 Key Laboratory of Biotherapy of Zhejiang Province, Biomedical Research Center, Sir Run Run Shaw Hospital, School of Medicine Zhejiang University, 3 Qingchun Road, Hangzhou, 310053, China
BMC Cancer 2012, 12:290 doi:10.1186/1471-2407-12-290Published: 16 July 2012
Additional file 1:
Figure S1. ZIC1 suppresses gastric cancer cell invasion. A BD Matrigel coated chamber was used to assess cell invasion. 1 × 105 AGS cells stably transfected with pCDNA3.1-ZIC1 or pCDNA3.1 empty vector were plated to the upper chamber and incubated for 24 h. Invaded cells were stained with Cell Stain solution, and detected on a standard microplate reader (560 nm). The relative invaded cells are expressed as the percentage rate compared with pcDNA3.1 empty vector transfectants (Bars, ±S.D. ; *p < 0.05).
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Additional file 2:
Figure S2. ZIC1 does not affect the cell apoptotic activity in gastric cancer cells. The cell apoptosis rate was determined by the Annexin V-PI flowcytometry assay after transient transfection with pCDNA3.1-ZIC1 or pCDNA3.1 empty vector in AGS and MKN28 cells for 24 h. Region LR indicates the percentage of early apoptotic cells, UR shows late apoptotic cells.
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Additional file 3:
Figure S3. Overexpression of ZIC1 inhibits the expression of Shh mRNA. AGS and SGC7901 cells were stably transfected with pCDNA3.1-ZIC1 or empty vector pCDNA3.1. The expression levels of ZIC1 and Shh mRNA were performed by RT-PCR. GAPDH was used as an internal control.
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