Open Access Research article

ZIC1 modulates cell-cycle distributions and cell migration through regulation of sonic hedgehog, PI3K and MAPK signaling pathways in gastric cancer

Jing Zhong1, Shujie Chen2, Meng Xue2, Qin Du1, Jianting Cai1, Hongchuan Jin3, Jianmin Si2 and Liangjing Wang1*

Author Affiliations

1 Department of Gastroenterology, Second Affiliated Hospital, School of Medicine Zhejiang University, 88 Jiefang Road, Hangzhou, 310009, China

2 Laboratory of Digestive Disease, Sir Run run Shaw Clinical Medicine Institution of Zhejiang University, 3 Qingchun Road, Hangzhou, 310053, China

3 Key Laboratory of Biotherapy of Zhejiang Province, Biomedical Research Center, Sir Run Run Shaw Hospital, School of Medicine Zhejiang University, 3 Qingchun Road, Hangzhou, 310053, China

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BMC Cancer 2012, 12:290  doi:10.1186/1471-2407-12-290

Published: 16 July 2012

Additional files

Additional file 1:

Figure S1. ZIC1 suppresses gastric cancer cell invasion. A BD Matrigel coated chamber was used to assess cell invasion. 1 × 105 AGS cells stably transfected with pCDNA3.1-ZIC1 or pCDNA3.1 empty vector were plated to the upper chamber and incubated for 24 h. Invaded cells were stained with Cell Stain solution, and detected on a standard microplate reader (560 nm). The relative invaded cells are expressed as the percentage rate compared with pcDNA3.1 empty vector transfectants (Bars, ±S.D. ; *p < 0.05).

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Additional file 2:

Figure S2. ZIC1 does not affect the cell apoptotic activity in gastric cancer cells. The cell apoptosis rate was determined by the Annexin V-PI flowcytometry assay after transient transfection with pCDNA3.1-ZIC1 or pCDNA3.1 empty vector in AGS and MKN28 cells for 24 h. Region LR indicates the percentage of early apoptotic cells, UR shows late apoptotic cells.

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Additional file 3:

Figure S3. Overexpression of ZIC1 inhibits the expression of Shh mRNA. AGS and SGC7901 cells were stably transfected with pCDNA3.1-ZIC1 or empty vector pCDNA3.1. The expression levels of ZIC1 and Shh mRNA were performed by RT-PCR. GAPDH was used as an internal control.

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